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The preparation of FTZ extract from eight constituent herbs was consistent with Aurora B inhibitor the protocol described previously, and as follows: Radix Salvia Miltiorrhiza, Radix Atractylodes Macrocephala, Fructus Citri Sarcodactylis, Cortex Eucommiae, and Herba Cirsii Jeponici had been extracted with boiling water twice, Fructus Ligustri Lucidi and Rhizoma Coptidis had been extracted with 70% ethanol twice, Radix Notoginseng was extracted with 50% ethanol twice.

20 lm lter, the ltrate Aurora B inhibitor was applied for UPLC analysis. All authentic standards were accurately weighed, and dissolved in methanol to obtain stock solutions with indicated concentrations. All the stock solutions were stored in the refrigerator at 4 C until analysis. Preparation of Serum Samples Capsule contents of FTZ, originated from the above extraction, were dispersed with distilled water as stock solution. The above suspension was orally administered to ve rats. An equal volume of distilled water was orally administered to the other ve rats as control, 30 min after drug administration, the animals were anaesthetized by ether inhalation. The blood was collected from the vena ophthalmica and then centrifuged at 10,000 rpm for 5 min at 4 C. The supernatant obtained was frozen immediately and stored at 80 C before use.

1. Fifty one peaks in FTZ were detected using UPLC?MS/MS, and 44 constituents were identied by comparing their retention behavior, the MS fragments characteristics to those of authentic standards. The names and structures of the identied constituents from Rhizoma Coptidis, Radix Notoginseng, Fructus Ligustri Lucidi, Radix Salvia miltiorrhiza, and other three herbs in both herbal HSP preparation and the serum samples for FTZ treated rats are listed in Tables 1, 2, 3, 4 and 5. The identied compounds are summarized in Table 6. In order to obtain MS fragmentation patterns of constituents in FTZ, MS2 spectra of 19 authentic standards were recorded by UPLC?MS/MS. Other peaks were identied, utilizing elemental composition analysis of their MS and MS2 data with software MassLynx from data and comparing with the literature data as well.

In the negative ion mode, ginsenosides, iridoid/secoiridoid glycosides, triterpene acids, and phenolic acids were observed in the FTZ, which originated from Radix Notoginseng, Fructus Ligustri Lucidi and Radix Salvia miltiorrhiza, respectively. Among them, six BI-1356 ginsenosides, peaks 38, were identied as notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rh1/F1 and ginsenoside Rb1 and ginsenoside Rd, respectively, by comparison with authentic standards and literature data. The mass spectra of the ginsenosides exhibited the molecular ion peaks at and. In the MS2 spectra, aglycone ions m/z 475 and 459 were nally formed by loss of several glycosidic units, which were the characteristic ions of panaxatriols and panaxadiols, respectively.

Peak 33 showed a molecular ion at m/z 1107 in MS spectra, m/z 945, m/z 783, m/z 603 and m/z 459 ions could be detected in the MS2 spectra, which exhibited a fragmentation pathway corresponding to the loss of glycosidic units.